If you are a researcher, a science student, or a laboratory expert, you must have heard about DNA Ladders. One of these ladders is the protein ladders that are used in experiments to estimate the proteins’ size. And gen electrophoresis is used to resolve it. The protein ladders contain a mixture of extremely purified proteins of known or defined molecular weights. These ladders have broader applications in biochemistry labs and life sciences. Two major types of protein ladders are prestained and unstained protein ladders. Let us know about them in detail in this article:
Prestained Protein ladders:
The prestained Protein ladders have protein mixtures that are prestained with the colors. They might have every protein prestained with one color, or they might have two or more proteins prestained with multiple colors, or every other protein prestained with different colors. Prestained protein ladders like BLUeye Prestained Protein Ladder come in three highly-intensified color reference bands of 75kDa, 50kDa, and 25kDa. All these bands help to monitor the migration or protein patterns and calculate the proteins’ molecular weights. The two types of prestained protein ladders are:
1. Prestained Recombinant Ladders:
When it comes to research use, these are the most preferred protein ladders. These ladders can produce evenly-spaced, sharp proteins prestained with multiple colors. It makes things simpler and easier to estimate the proteins’ approximate molecular weight. For example, the 100bp DNA Ladder H3 RTU (Ready-to-Use) is a unique combination of PCR products. It makes things easy when it comes to the estimation of weights.
2. Prestained Naturally-Occurring Ladders:
These proteins make broad bands when stained with 1 or more colors and show the uneven amount and location of the dies that bind every protein. The researchers trying to make indistinct broad bands may not use this protein to fulfill their problem.
Unstained Protein Ladders:
The unstained protein ladders are a mixture of proteins that are not stained with any sort of color. These proteins facilitate the formation of identifiable sharp bands in gels. It leads to the correct estimation of proteins’ molecular weights. The unstained protein ladder facilitates a direct estimate of the proteins. This is because it is different from prestained protein ladders like BLUelf Prestained Protein Ladder and their gel matrix is not blocked by the dyes’ binding. The ladder bands remain sharp and allow every precise molecular weight determination of the proteins in the experimental sample.
The unstained protein ladders are the ladders in which the polypeptides or bands are not prestained. As a result, it can be visualized while the gel runs. However, the visualization occurs after the total gel is stained with a dye like Coomassie. Because of no additional stain is included during the electrophoresis, the representative weight of the polypeptide is more accurate. The size that each band of the ladder represents is solely the corresponding polypeptide constituent’s weight.
Conclusion:
The prestained protein ladders and the unstained protein ladders are the most in-demand protein ladders. However, there are a few more that are not used like these two. If you are looking for the best protein ladders at effective prices, you may visit Unibiotech and choose the right protein ladder to get your job done.
Comments